abstract：:The visible and near-uv absorption and circular dichroic spectra were determined for spinach and poplar plastocyanin under a variety of conditions. The visible spectra showed that the copper center was invariant to changes in species, chemical modification with ethylenediamine, and addition of high concentrations of s...

journal_title：Archives of biochemistry and biophysics

authors： Draheim JE,Anderson GP,Pan RL,Rellick LM,Duane JW,Gross EL

更新日期：1985-02-15 00:00:00

abstract：:NAD kinase from human neutrophils has been partially purified by sequential application of Red Agarose, ion-exchange, and gel-filtration chromatography. The enzyme has a broad pH optimum, 7.0-9.5, is strictly dependent upon the presence of Mg2+, and in the absence of calcium exhibits Km values of 0.6 and 0.9 mM for NA...

journal_title：Archives of biochemistry and biophysics

authors： Williams MB,Jones HP

更新日期：1985-02-15 00:00:00

abstract：:A monoclonal antibody specific for cytochrome P-450 1 that extensively (greater than 95%) inhibits the hepatic 21-hydroxylation of progesterone was used in a two-site immunoradiometric assay to estimate the concentration of cytochrome P-450 1 in microsomes prepared from 24 individual, untreated New Zealand White rabbi...

journal_title：Archives of biochemistry and biophysics

authors： Johnson EF,Griffin KJ

更新日期：1985-02-15 00:00:00

abstract：:The various interactions of rat liver dihydrofolate reductase with two unconjugated 7,8-dihydropteridines, 7,8-dihydrobiopterin and 6-methyl-7,8-dihydropteridine, have been compared with those of 7,8-dihydrofolate and folate. Of particular interest was the reactivity demonstrated by 7,8-dihydrobiopterin because of the...

journal_title：Archives of biochemistry and biophysics

authors： Webber S,Whiteley JM

更新日期：1985-02-01 00:00:00

abstract：:Glucosylsphingosine is a potent inhibitor of lysosomal glucocerebrosidase and the broad-specificity, cytosolic beta-glucosidase of human liver. In the present study, it was demonstrated that the broad-specificity beta-glucosidase was also inhibited by galactosylsphingosine. The inhibition was observed when the enzyme ...

journal_title：Archives of biochemistry and biophysics

authors： LaMarco KL,Glew RH

更新日期：1985-02-01 00:00:00

abstract：:Mitoplasts were prepared from 3-h ischemic livers in an attempt to define the structural alterations in the inner membrane that may account for the functional deficiencies of ischemic mitochondria. Mitoplasts from both control and ischemic livers had similar specific activities of cytochrome oxidase and succinate-cyto...

journal_title：Archives of biochemistry and biophysics

authors： Okayasu T,Curtis MT,Farber JL

更新日期：1985-02-01 00:00:00

abstract：:Using ammonium sulfate precipitation, gel filtration, and affinity chromatography, inosine monophosphate (IMP) oxidoreductase (EC 1.2.1.14) was isolated from the soluble proteins of the plant cell fraction of nitrogen-fixing nodules of cowpea (Vigna unguiculata L. Walp). The enzyme, purified more than 140-fold with a ...

journal_title：Archives of biochemistry and biophysics

authors： Atkins CA,Shelp BJ,Storer PJ

更新日期：1985-02-01 00:00:00

abstract：:Serine is an essential amino acid for the lectin-mediated transformation of human peripheral blood lymphocytes due to the inability of this cell to synthesize sufficient quantities via either the phosphorylated pathway or by reversal of the serine hydroxymethyltransferase reaction to meet the metabolic demands. The le...

journal_title：Archives of biochemistry and biophysics

authors： Rowe PB,Sauer D,Fahey D,Craig G,McCairns E

更新日期：1985-01-01 00:00:00

abstract：:Interactions of six carbohydrates (trehalose, sucrose, glucose, raffinose, inositol, and glycerol) with dry dipalmitoylphosphatidylcholine (DPPC) were studied using differential scanning calorimetry (DSC) and infrared spectroscopy (ir) in order to elucidate the mechanism by which some of these carbohydrates preserve s...

journal_title：Archives of biochemistry and biophysics

authors： Crowe LM,Crowe JH,Chapman D

更新日期：1985-01-01 00:00:00

abstract：:Swainsonine, a plant toxin, strongly inhibits certain alpha-D-mannosidases but has no effect on others [D. R. P. Tulsiani, T. M. Harris, and O. Touster (1982) J. Biol. Chem. 257, 7936-7939]. The reversible inhibition of jack bean and lysosomal alpha-D-mannosidases has previously been suggested to be similar in nature ...

journal_title：Archives of biochemistry and biophysics

authors： Tulsiani DR,Broquist HP,Touster O

更新日期：1985-01-01 00:00:00

abstract：:The nucleotide sequence of tyrosine tRNAQ* psi A from bovine liver was determined to be pC-C-U-U-C-m2G-A-U-A-m2G-C-U-C-A-G-D-D-G-G-acp3U-A-G-A -G-C-m22G-m22G -A-G-G-A-C-U-Q*-psi-A-m1G-A-psi m-C-C-U-U-A-G-m7G-D-m5C-G-C-U-G-G-T-psi-C-G-m1A -U-U-C-C-G-G-C-U-C-G-A-A-G-G-A-C-C-AOH. This tyrosine tRNA is 76 nucleotides in l...

journal_title：Archives of biochemistry and biophysics

authors： Johnson GD,Pirtle IL,Pirtle RM

更新日期：1985-01-01 00:00:00

abstract：:The insulin receptor from rat skeletal muscle was characterized. Treatment of muscle membranes with the photoactive insulin analog, 125I[N-epsilonB29-monoazidobenzoyl]-insulin revealed a single protein band of 135,000 Da, the alpha subunit. Iodination of total membrane protein followed by Triton X-100 solubilization a...

journal_title：Archives of biochemistry and biophysics

authors： Dubler RE,Whipple JH,Larner J

更新日期：1985-01-01 00:00:00

abstract：:The effect of transition from a respiring to a respiration-inhibited state on the rate of protein synthesis was investigated in glycolyzing, cultured rat heart cells. The rate was found to be significantly lower after blocking respiration, and it was further decreased by L-lactate. In contrast, pyruvate or phenazine m...

journal_title：Archives of biochemistry and biophysics

authors： Ravid K,Diamant P,Avi-Dor Y

更新日期：1985-01-01 00:00:00

abstract：:The velocity of acetaldehyde metabolism in rat liver may be governed either by the rate of regeneration of NAD from NADH through the electron transport system or by the activity of aldehyde dehydrogenase (ALDH). Measurements of oxygen consumption revealed that the electron transport system was capable of reoxidizing A...

journal_title：Archives of biochemistry and biophysics

authors： Svanas GW,Weiner H

更新日期：1985-01-01 00:00:00

abstract：:Incubation of rat liver nuclear envelopes with [gamma-32P]ATP resulted in the synthesis of phosphatidylinositol-[4-32P]phosphate (PIP). Degradation of endogenously labeled PIP was observed upon the dilution of the labeled ATP with an excess of unlabeled ATP. This degradation was most rapid in the presence of EDTA, and...

journal_title：Archives of biochemistry and biophysics

authors： Smith CD,Wells WW

更新日期：1984-12-01 00:00:00

abstract：:Pretreatment of rabbits with imidazole resulted in a twofold increase in hepatic microsomal cytochrome P-450 content, with the apparent induction of two or more distinct forms of the cytochrome [K. K. Hajek and R. F. Novak (1982) Biochem. Biophys. Res. Commun. 108, 664-672]. The metabolic properties of imidazole-induc...

journal_title：Archives of biochemistry and biophysics

authors： Kaul KL,Novak RF

更新日期：1984-12-01 00:00:00

abstract：:Eleven hybridoma clones which secrete monoclonal antibodies against purified rat liver alcohol dehydrogenase (EC 1.1.1.1) were isolated. Antibodies (R-1-R-11) were identified by their ability to bind to immobilized pure alcohol dehydrogenase in an enzyme-linked immunoadsorbent assay, in which antibody R-9 showed the h...

journal_title：Archives of biochemistry and biophysics

authors： Lad PJ,Schenk DB,Leffert HL

更新日期：1984-12-01 00:00:00

abstract：:A technique is presented for the preparation of three major proteoglycans from 14-day embryonic chicken retinas following their culture overnight with [35S]sulfate and either [3H]glucosamine or [3H]serine. Homogenization of the tissue in saline permitted extraction of heterogeneous soluble proteoglycans separately fro...

journal_title：Archives of biochemistry and biophysics

authors： Morris JE

更新日期：1984-11-15 00:00:00

abstract：:A 1.1-kb Hp alpha I fragment of the Escherichia coli chromosome containing the gene for translation initiation factor 3 was employed as a probe in heterologous hybridization to chromosomal DNA from a variety of other procaryotes. Positive hybridization was observed to DNA derived from all gram-negative bacteria tested...

journal_title：Archives of biochemistry and biophysics

authors： Pramanik A,Schwartz I

更新日期：1984-11-15 00:00:00

abstract：:Phenylalanine ammonia-lyase forms trans-cinnamate from L-phenylalanine, and thus stands at a gateway to secondary metabolism in higher plants. L-alpha-Amino-oxy-beta-phenylpropanoic acid (L-AOPP), a very effective competitive inhibitor of this enzyme, is most probably a transition-state analog for the elimination reac...

journal_title：Archives of biochemistry and biophysics

authors： Jones DH,Northcote DH

更新日期：1984-11-15 00:00:00

abstract：:The in vivo turnover of several rat liver microsomal proteins was studied using techniques designed to maximize antibody recognition specificity and minimize reutilization of radioactive labels. The kinetics of degradation of seven cytochrome P-450 isozymes, NADPH-cytochrome P-450 reductase, and epoxide hydrolase were...

journal_title：Archives of biochemistry and biophysics

authors： Shiraki H,Guengerich FP

更新日期：1984-11-15 00:00:00

abstract：:Estrogen-induced chick oviduct differentiation is accompanied by an increased capacity for protein glycosylation. A portion of this increase has been attributed to increased levels of dolichyl phosphate (Dol-P). Hormone withdrawal leads to an apparent decrease in Dol-P. Dol-P metabolism in the oviduct has been studied...

journal_title：Archives of biochemistry and biophysics

authors： DeRosa PA,Lucas JJ

更新日期：1984-11-01 00:00:00

abstract：:The effects of pH, NaCl, and malate2- on the equilibrium between dimeric and higher-molecular-weight forms of NAD malic enzyme from Solanum tuberosum var. Chieftain have been analyzed by monitoring the kinetic changes associated with disaggregation [S. D. Grover and R. T. Wedding (1982) Plant Physiol. 70, 1169-1172]. ...

journal_title：Archives of biochemistry and biophysics

authors： Grover SD,Wedding RT

更新日期：1984-11-01 00:00:00

abstract：:The in vivo stability of ferredoxin-nitrate reductase from the cyanobacterium Anacystis nidulans under conditions of inhibited protein synthesis has been studied in nitrate-grown cells. A light-promoted rapid decay in cellular nitrate reductase activity took place in the absence of any added nitrogen source, but not i...

journal_title：Archives of biochemistry and biophysics

authors： Herrero A,Flores E,Guerrero MG

更新日期：1984-11-01 00:00:00

abstract：:Diphtheria toxin (DT) induces the leakage of dipalmitoylphosphatidic acid (DPPA) membranes but not neutral dipalmitoylphosphatidylcholine (DPPC) membranes. Cholesterol incorporated into liposomes enhances the membrane leakage induced by DT in acidic DPPA membranes but not in neutral DPPC membranes. Membrane leakage wa...

journal_title：Archives of biochemistry and biophysics

authors： Lai CS,Kushnaryov V,Panz T,Basosi R

更新日期：1984-10-01 00:00:00

abstract：:Phospholipid transmethylation in the microsomal fraction of stimulated and unstimulated human leukocytes was measured in a recently developed assay system. Microsomal fraction was prepared from neutrophils, unseparated lymphocytes, T lymphocytes, and non-T lymphocytes by sonication and subsequent ultracentrifugation. ...

journal_title：Archives of biochemistry and biophysics

authors： Niwa Y,Sakane T,Taniguchi S

更新日期：1984-10-01 00:00:00

abstract：:A comparative study was made of the metal ion requirement of rat liver mitochondrial phospholipase A2 in purified and membrane-associated forms. Membrane-bound enzyme was assayed using either exogenous or endogenous phosphatidylethanolamine. Although several divalent metal ions caused increased activity of the membran...

journal_title：Archives of biochemistry and biophysics

authors： De Winter JM,Korpancova J,van den Bosch H

更新日期：1984-10-01 00:00:00

abstract：:Effects of free fatty acids on parameters of (Na+,K+)-ATPase regulation related to enzyme conformation were examined. Sensitivity to inhibition by free fatty acid increased as the number of double bonds increased. Free fatty acids reduced affinity for K+ or Na+ at their regulatory sites without altering apparent K+ af...

journal_title：Archives of biochemistry and biophysics

authors： Swann AC

更新日期：1984-09-01 00:00:00

abstract：:The single-stranded, DNA-dependent ATPase activity of purified recA protein was found to be inhibited competitively by ribose-modified analogs of ATP, 3'-O-anthraniloyl-ATP (Ant-ATP), and 3'-O-(N-methylanthraniloyl)-ATP (Mant-ATP). The Ki values for Ant-ATP and Mant-ATP were around 7 and 3 microM at pH 7.5, respective...

journal_title：Archives of biochemistry and biophysics

authors： Karasaki Y,Higashi K

更新日期：1984-09-01 00:00:00

abstract：:Previous work has shown that the coupling of the soluble Escherichia coli pyruvate oxidase to a lipid-depleted membrane terminal electron transport system requires the addition of ubiquinone and a neutral lipid fraction (C. Cunningham and L. P. Hager (1975) J. Biol. Chem. 250, 7139-7146). The active factor present in ...

journal_title：Archives of biochemistry and biophysics

authors： Kiuchi K,Hager LP

更新日期：1984-09-01 00:00:00

abstract：:Liver mitochondria provided with an oxidizable substrate, ATP, oxygen, and an ADP-generating system (soluble F1-ATPase) were used to reevaluate the rate-controlling step(s) intrinsic to all of the processes of mitochondrial oxidative phosphorylation. The quantity termed "control strength" (C), previously defined as th...

journal_title：Archives of biochemistry and biophysics

authors： Davis EJ,Davis-Van Thienen WI

更新日期：1984-09-01 00:00:00

abstract：:The effects of in vitro addition of halogenated hydrocarbons on the susceptibility of various rat tissues to lipid peroxidation, and of iron overload and dietary vitamin E in the intact rat on subsequent lipid peroxidation in rat tissue slices were examined. The ease and speed of tissue slice preparation allowed testi...

journal_title：Archives of biochemistry and biophysics

authors： Gavino VC,Dillard CJ,Tappel AL

更新日期：1984-09-01 00:00:00

abstract：:Steady-state kinetic studies of the bovine carbonic anhydrase B-catalyzed hydration of CO2, dehydration of HCO3-, and hydrolysis of p-nitrophenylacetate were made in glycerol/water solvents of increased viscosity in order that the effect of diffusion-control on the substrate association reactions could be determined. ...

journal_title：Archives of biochemistry and biophysics

authors： Hasinoff BB

更新日期：1984-09-01 00:00:00

abstract：:Incubation of Novikoff rat hepatoma cells; mouse L929, P388 and L1210 cells; and Chinese hamster ovary cells with sulfhydryl reagents, such as p-hydroxymercuribenzoate or p-hydroxymercuribenzenesulfonate, reduced the zero-trans influx of uridine in a concentration-dependent manner. The sensitivity of uridine transport...

journal_title：Archives of biochemistry and biophysics

authors： Plagemann PG,Wohlhueter RM

更新日期：1984-09-01 00:00:00

abstract：:The effect of phenothiazines (trifluoperazine, chlorpromazine, methochlorpromazine, and imipramine) on Ca2+ fluxes in light and heavy sarcoplasmic reticulum (SR) isolated from rabbit fast-twitch skeletal muscle was investigated. These drugs inhibited Ca2+ loading and (Ca2+,Mg2+)-ATPase activity, but had no effect on u...

journal_title：Archives of biochemistry and biophysics

authors： Volpe P,Costello B,Chu A,Fleischer S

更新日期：1984-08-15 00:00:00

abstract：:This paper describes the effect of an ionic (sodium dodecyl sulfate; SDS) and a nonionic detergent (Triton X-100) on the substrate and enzyme components of the lecithin: cholesterol acyltransferase (LCAT) reaction. When the enzyme sources (purified or partially purified) or the respective substrates [high-density lipo...

journal_title：Archives of biochemistry and biophysics

authors： Park MC,McConathy WJ,Lacko AG

更新日期：1984-08-15 00:00:00

abstract：:Butyric acid produces multiple effects on mammalian cells in culture, including alterations in morphology, depression of growth rate, increased histone acetylation, and modified production of various proteins and enzymes. The latter effect is exemplified by the induction in HeLa cells of the glycoprotein hormone alpha...

journal_title：Archives of biochemistry and biophysics

authors： McClure DS,Cox GS

更新日期：1984-08-15 00:00:00

abstract：:A monoclonal antibody raised against rabbit liver cathepsin M binds to intact rabbit liver lysosomes. The binding is specific and is abolished by treating the lysosomes with trypsin, which has previously been shown to digest the membrane-bound cathepsin M [S. Pontremoli, E. Melloni, M. Michetti, F. Salamino, B. Sparat...

journal_title：Archives of biochemistry and biophysics

authors： Pontremoli S,Melloni E,Damiani G,Michetti M,Salamino F,Sparatore B,Horecker BL

更新日期：1984-08-15 00:00:00

abstract：:Some properties and subcellular localization of adenosine diphosphatase (ADPase) activity from rat heart have been investigated. The pH optimum was 7.4, maximal activity was found with 5 mM MgCl2, and the apparent Km was 20 microM. ADPase activity was strongly inhibited by NaF and AppNHp, and to a lesser extent by AMP...

journal_title：Archives of biochemistry and biophysics

authors： de Vente J,Velema J,Zaagsma J

更新日期：1984-08-15 00:00:00

abstract：:The spectral properties of 4-methylumbelliferyl-glycosides (MeUmb-glycosides) were investigated in order to assess their usefulness as probes of the microenvironment of sugar binding sites on lectin molecules. It was shown that the abnormally high values for fluorescence polarization of free MeUmb-glycosides (from 0.0...

journal_title：Archives of biochemistry and biophysics

authors： Decastel M,Vincent M,Matta KL,Frénoy JP

更新日期：1984-08-01 00:00:00